Coombs Test


What is a Coombs test?

A Coombs test checks for antibodies that can attack your red blood cells. Antibodies are a vital part of your immune system. They fight off antigens (like bacteria, viruses and other toxins) to keep you healthy. But sometimes antibodies mistakenly attack normal, healthy cells instead.

What is the Coombs test used for?

First, it’s important to note that there are two different types of Coombs tests: direct and indirect.

  • Direct Coombs test (sometimes called a direct antiglobulin test) looks at your red blood cells to see if there are antibodies attached to them. It’s often used to diagnose blood-related conditions such as autoimmune hemolytic anemia. People with autoimmune hemolytic anemia don’t have enough red blood cells because their body destroys them too soon.
  • Indirect Coombs test tells your provider if there are antibodies present in your bloodstream that could attach to red blood cells. It’s used as a screening process to see how you’ll react to a blood transfusion. An indirect Coombs test is also routinely given as part of prenatal testing.

Coombs test during pregnancy

An indirect Coombs test is given to every pregnant person to see if they have antibodies against Rh-positive blood. For example, if you have an Rh-negative blood type (such as O-, A-, B- or AB-), your red blood cells don’t have the Rh factor marker on them. But people with Rh-positive blood types do have this specific marker.

If an Rh-negative person is pregnant with an Rh-positive fetus (developing baby), then Rh sensitization can occur. This means that your immune system will mistakenly target the Rh factor as a threat and make antibodies to destroy it. It can take a long time for your body to create antibodies that could harm your baby. Therefore, during your first pregnancy, your baby probably wouldn’t be affected. However, if you get pregnant again with an Rh-positive baby, the existing antibodies in your blood could attack your baby’s red blood cells. This can result in Rh disease, which is characterized by anemia, jaundice or other serious health problems. These medical issues usually worsen with every Rh-positive pregnancy you have.

If you’re at risk for Rh sensitization, then the problem can almost always be prevented with treatment. If you’re already sensitized, then treatment can help protect your baby. That’s why it’s so important to perform the Coombs test during your first trimester.

Coombs test for newborn babies

If your baby is born with jaundice, then your healthcare provider will likely perform a Coombs test. This helps detect any “foreign” antibodies that have attached to your newborn’s red blood cells.

Test Details

How does the Coombs test work?

To perform the Coombs test, your healthcare provider will need to take a small sample of your blood.

How do I prepare for a Coombs test?

No preparation is necessary before a Coombs test.

What can I expect during the test?

Your healthcare provider uses a small needle to take a sample of blood from a vein in your arm or hand. You may feel a slight “pinch” or “prick” on your skin when the needle goes in. Once the sample is collected, it’s sent to a lab for analysis. Your provider will put a bandage over the location where the needle was placed. You can remove the bandage after 10 to 20 minutes.

Results and Follow-Up

When should I know the results of the test?

In most cases, you’ll find out the results of your test within 24 hours.

What does a positive Coombs test mean?

A positive (abnormal) Coombs test means that you have antibodies that can attack your red blood cells. This can be caused by several conditions, including:

What does a positive test before a blood transfusion mean?

If you test positive before a blood transfusion, it means that your healthcare provider needs to use caution when choosing a donor. People who receive a lot of blood transfusions usually develop several different antibodies. As a result, they may have trouble finding blood that will work.

What does a positive test during pregnancy mean?

If you test positive during your pregnancy, it means you’ll need to take steps to protect your baby from Rh sensitization.

What does Coombs positive mean for a baby?

Generally a positive Coombs test result indicates anemia or jaundice. If your baby tests positive, then your healthcare provider will perform an examination and recommend appropriate treatment.

What does a negative Coombs test result mean?

A negative (normal) test result is good news. It means that no antibodies to red blood cells were found. Depending on your situation, it means that you:

  • Can safely receive blood from a donor during a transfusion.
  • Don’t have to worry about Rh sensitization affecting your unborn baby.

When should I call my healthcare provider?

If you develop symptoms of hemolytic anemia, such as jaundice, weakness, dizziness or confusion, call your healthcare provider right away. They can perform an examination and run tests to determine the appropriate treatment.

Many common warning signs can overlap with symptoms of other diseases and conditions. If you notice that something isn’t quite right, schedule an appointment with your provider.

A note from Cleveland Clinic

The Coombs test looks for antibodies that may attack and destroy your red blood cells. Your healthcare provider must obtain a sample of your blood to perform the analysis. While Coombs test results can point your medical team in the right direction, you may need further testing to find the right diagnosis and treatment.

Last reviewed by a Cleveland Clinic medical professional on 05/11/2022.


  • National Library of Medicine. Coombs test. ( Accessed 5/11/2022.
  • The American College of Obstetricians and Gynecologists. The Rh Factor: How It Can Affect Your Pregnancy. ( Accessed 5/11/2022.
  • Theis SR, Hashmi MF. Coombs Test. ( [Updated 2021 Sep 16]. In: StatPearls [Internet]. Treasure Island (FL): StatPearls Publishing; 2021 Jan-. Accessed 5/11/2022.

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